Primer-BLAST

Primer-BLAST

NCBI

4.7( reviews)
PCR
Primer Design
Specificity Check

About

Learn more about this tool

A tool for designing target-specific primers that combines Primer3 with BLAST to ensure specificity. It helps avoid amplification of non-target sequences.

Key Features

  • Specificity checking
  • Multiple template support
  • Exon/intron junction spanning
  • SNP awareness

Technical Specifications

Platform
License
Last Updated
2023-09-22T00:00:00+00:00

Performance Metrics

Evaluation and benchmarks

Performance Score85%

This tool demonstrates high accuracy in predicted results compared to experimental validation.

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